Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF444
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 200-250 of Human ZNF44 (NP_060807.2).

  • Positive control
    • HeLa, Jurkat and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab117765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Transcriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endothelial zinc finger protein 2 antibody
    • EZF 2 antibody
    • EZF-2 antibody
    • EZF2 antibody
    • FLJ11137 antibody
    • ZCCAN17 antibody
    • Zinc finger and SCAN domain-containing protein 17 antibody
    • Zinc finger protein 444 antibody
    • ZN444_HUMAN antibody
    • ZNF444 antibody
    • ZSCAN17 antibody
    see all

Images

  • All lanes : Anti-ZNF444 antibody (ab117765) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 30 seconds
  • Detection of ZNF444 by Western Blot of Immunprecipitate.
    ab117765 at 1µg/ml staining ZNF444 in HeLa whole cell lysate immunoprecipitated using ab117765 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab117765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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