Overview

  • Product nameAnti-ZNF444 antibody
    See all ZNF444 primary antibodies
  • Description
    Rabbit polyclonal to ZNF444
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human ZNF444 according to NP_060807.2.

  • Positive control
    • Whole cell lysate from HeLa, 293T and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab117812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionTranscriptional regulator. Binds to the 5'-flanking critical region of the SCARF1 promoter.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endothelial zinc finger protein 2 antibody
    • EZF 2 antibody
    • EZF-2 antibody
    • EZF2 antibody
    • FLJ11137 antibody
    • ZCCAN17 antibody
    • Zinc finger and SCAN domain-containing protein 17 antibody
    • Zinc finger protein 444 antibody
    • ZN444_HUMAN antibody
    • ZNF444 antibody
    • ZSCAN17 antibody
    see all

Anti-ZNF444 antibody images

  • All lanes : Anti-ZNF444 antibody (ab117812) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 30 seconds
  • ab117812 at 1 µg/ml staining ZNF444 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab117812 at 6 µg/mg lysate.
    Lane 2; IP using an antibody to a downstream epitope.
    Lane 3; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.

References for Anti-ZNF444 antibody (ab117812)

ab117812 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"