Anti-ZNF462 antibody (ab117771)
Key features and details
- Rabbit polyclonal to ZNF462
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF462 antibody -
Description
Rabbit polyclonal to ZNF462 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human ZNF462 aa 2450-2550.
Database link: Q96JM2 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab117771 was affinity purified using an epitope specific to ZNF462 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 285 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 285 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in transcriptional regulation. -
Sequence similarities
Contains 27 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 58499 Human
- SwissProt: Q96JM2 Human
- Unigene: 370379 Human
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Alternative names
- DKFZp686B2325 antibody
- DKFZp762N2316 antibody
- FLJ14960 antibody
see all
Images
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All lanes : Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 285 kDaSamples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemical analysis of human lung cancer tissue labeling ZNF462 with ab117771 at a dilution of 1/5000.
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Detection of human ZNF462 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for IP at 6 µg per reaction. For blotting immunoprecipitated ZNF462, ab117771 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Lane 1 : Anti-ZNF462 antibody (ab117771)
Lane 2 : Control IgG
All lanes : -
Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 285 kDaSamples: Whole cell lysate (50 µg) from NIH/3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified ab117771 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab117771 has been referenced in 1 publication.
- Devkota S et al. Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy 12:2038-2053 (2016). PubMed: 27541728