Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF462
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 2456-2506 of Human ZNF462 (NP_067047.4).

  • Positive control
    • 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117771 was affinity purified using an epitope specific to ZNF462 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 285 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Contains 27 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686B2325 antibody
    • DKFZp762N2316 antibody
    • FLJ14960 antibody
    • FLJ45904 antibody
    • KIAA1803 antibody
    • RP11-508N12.1 antibody
    • Zinc finger protein 462 antibody
    • ZN462_HUMAN antibody
    • ZNF462 antibody
    see all

Images

  • All lanes : Anti-ZNF462 antibody (ab117771) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg

    Developed using the ECL technique

    Predicted band size : 285 kDa


    Exposure time : 3 minutes
  • Detection of ZNF462 by Western Blot of Immunprecipitate.
    ab117771 at 1µg/ml staining ZNF462 in 293T whole cell lysate immunoprecipitated using ab117771 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Devkota S  et al. Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy 12:2038-2053 (2016). Read more (PubMed: 27541728) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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