The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay be involved in transcriptional regulation.
Tissue specificityHighly expressed in testis. Very low expression in thymus. No expression in other tissues tested. Expressed in a differentiation stage-specific manner in mesenchymal chondrogenic progenitor cells.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 17 C2H2-type zinc fingers. Contains 1 KRAB domain.