Overview

  • Product name
    Anti-ZNF48 antibody
  • Description
    Rabbit polyclonal to ZNF48
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GLGSENVISQ PNEFEHTPQE DDLGFKEEDL APDHEVGNVS LKPEGIQNWD DLWVQREGLG KPQPRDRGPR LLGEPRWGQA SSDRA, corresponding to amino acids 27-111 of Human ZNF48 (Q96MX3).

  • Positive control
    • Human kidney tissue; RT-4 cell lysate; U-251 MG cell lysate; Human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab121776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 12 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp762K013 antibody
    • FLJ31751 antibody
    • MGC43952 antibody
    • Zinc finger protein 48 antibody
    • Zinc finger protein 553 antibody
    • Znf48 antibody
    • ZNF48_HUMAN antibody
    • ZNF553 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121776 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ZNF48 antibody (ab121776) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab121776 at a 1/50 dilution staining ZNF48 in Human kidney tissue (paraffin embedded) by immunohistochemistry.

References

ab121776 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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