Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF485
  • Specificity
    This antibody reacts with ZNF485
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: RHSSGLVEHQ RLHTGEKPYK CNECGKAFPR SSALKQHKKI HNKERAMKCS, corresponding to amino acids 392-441 of Human ZNF485

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Zinc finger protein 485 antibody
    • ZN485_HUMAN antibody
    • ZNF485 antibody

Images

  • Lane 1 :
    Lane 2 : Anti-ZNF485 antibody (ab50559) at 0.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 46 kDa
    Observed band size : 35,50 kDa (why is the actual band size different from the predicted?)

References

ab50559 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50559.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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