Overview

  • Product name
    Anti-ZNF485 antibody
    See all ZNF485 primary antibodies
  • Description
    Rabbit polyclonal to ZNF485
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with ZNF485
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: RHSSGLVEHQ RLHTGEKPYK CNECGKAFPR SSALKQHKKI HNKERAMKCS, corresponding to amino acids 392-441 of Human ZNF485

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.

Titre using peptide based assay: 1/62500.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Zinc finger protein 485 antibody
      • ZN485_HUMAN antibody
      • ZNF485 antibody

    Images

    • Western blot - Anti-ZNF485 antibody (ab50559)
      Western blot - Anti-ZNF485 antibody (ab50559)
      Lane 2 : Anti-ZNF485 antibody (ab50559) at 0.25 µg/ml

      Lane 1 : MARKER
      Lane 2 : HepG2 cell lysate at 10 µg

      Secondary
      Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 46 kDa
      Observed band size: 35,50 kDa (why is the actual band size different from the predicted?)

    References

    ab50559 has not yet been referenced specifically in any publications.

    Publishing research using ab50559? Please let us know so that we can cite the reference in this datasheet.

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