- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-ZNF485 antibody
See all ZNF485 primary antibodies - DescriptionRabbit polyclonal to ZNF485
- SpecificityThis antibody reacts with ZNF485
- Tested applicationsSuitable for: ELISA, WBmore details
- Species reactivityReacts with: Human
- Immunogen
A region within synthetic peptide: RHSSGLVEHQ RLHTGEKPYK CNECGKAFPR SSALKQHKKI HNKERAMKCS, corresponding to amino acids 392-441 of Human ZNF485
- Positive control
- HepG2 cell lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/62500. Titre using peptide based assay: 1/62500. |
|
| WB | Use a concentration of 0.25 µg/ml. Detects a band of approximately 50, 35 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionMay be involved in transcriptional regulation.
- Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 11 C2H2-type zinc fingers.
Contains 1 KRAB domain. - Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 220992 Human
- SwissProt: Q8NCK3 Human
- Unigene: 147440 Human
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Alternative names
- Zinc finger protein 485 antibody
- ZN485_HUMAN antibody
- ZNF485 antibody
Anti-ZNF485 antibody images
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Western blot - ZNF485 antibody (ab50559)Lane 1 :
Lane 2 : Anti-ZNF485 antibody (ab50559) at 0.25 µg/ml
Lane 1 : MARKER
Lane 2 : HepG2 cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 46 kDa
Observed band size : 35,50 kDa (why is the actual band size different from the predicted?)
References for Anti-ZNF485 antibody (ab50559)
ab50559 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"