Overview

  • Product nameAnti-ZNF498 antibody
    See all ZNF498 primary antibodies
  • Description
    Rabbit polyclonal to ZNF498
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide: DCFGEYVEPQ DCRVSPGGGS KEKEAKPPQE DLKGALVALT SERFGEASLQ, corresponding to amino acids 253-302 of Human ZNF498

  • Positive control
    • Jurkat cell lysate, human intestinal tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

Target

Anti-ZNF498 antibody images

  • Anti-ZNF498 antibody (ab58700) at 1.25 µg/ml + Jurkat cell extract at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/100,000 dilution.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling ZNF498 with ab58700 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of intestinal villi. Magnification: 400X.

References for Anti-ZNF498 antibody (ab58700)

ab58700 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"