Overview

  • Product nameAnti-ZNF499 antibody
    See all ZNF499 primary antibodies
  • Description
    Rabbit polyclonal to ZNF499
  • Specificityab28460 recognises zinc finger protein 499 (ZNF499).
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cat, Dog
  • Immunogen

    Synthetic peptide derived from residues 200 - 249 of human ZNF499.

    .

  • Positive control
    • Jurkat cell lysate, human kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab28460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.06 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.

Target

  • RelevanceZinc finger proteins are DNA and RNA binding proteins that contain motifs where amino acids are folded into a single structural unit around a zinc atom. In a standard zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Zinc finger and BTB domain-containing protein 45 antibody
    • zinc finger protein 499 antibody

Anti-ZNF499 antibody images

  • Anti-ZNF499 antibody (ab28460) at 0.06 µg/ml

    Predicted band size : 54 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    ZNF449 detected in Jurkat cell lysate. Gel concentration was 12%. A secondary anti-rabbit HRP-conjugated has been used (suggested dilution: 1:50000-100000)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ZNF499 with ab28460 at 4-8µg/ml. Cells with positive labels are indicated by the arrows (epithelial cells of collecting tubule). Magnification: 400X.

References for Anti-ZNF499 antibody (ab28460)

ab28460 has not yet been referenced specifically in any publications.

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