Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF499
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CEEPPAPTGL ADYSGAGRDF LRGAGSAEDV FPDSYVSTWH DEDGAVPEGC , corresponding to Internal sequence amino acids 252-302 of Human ZNF499

  • Positive control
    • Fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab42431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.015 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • Relevance
    Zinc finger proteins are DNA and RNA binding proteins that contain motifs where amino acids are folded into a single structural unit around a zinc atom. In a standard zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Zinc finger and BTB domain-containing protein 45 antibody
    • zinc finger protein 499 antibody

Images

  • Anti-ZNF499 antibody (ab42431) at 0.015 µg/ml + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)

References

ab42431 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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