Anti-ZNF511 antibody (ab87137)

Overview

  • Product nameAnti-ZNF511 antibody
    See all ZNF511 primary antibodies
  • Description
    Rabbit polyclonal to ZNF511
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (QLPPALCARL AAGPGAAEPL PVERDPAAGA APFRFVARPV RFPREHQFFE) of Human ZNF511 (NP_665805).

  • Positive control
    • Transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ZNF511 antibody images

  • Anti-ZNF511 antibody (ab87137) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cel lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : ~35 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF511 antibody (ab87137)

ab87137 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - whole cell (HEK293T)
Specification HEK293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Sep 22 2014

Thank you for contacting us. I am sorry to confirm that because we carry over 80,000 products, it is not feasible for us to keep small sample sizes of our products.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"