Anti-ZNF511 antibody (ab87137)

Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF511
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (QLPPALCARL AAGPGAAEPL PVERDPAAGA APFRFVARPV RFPREHQFFE) of Human ZNF511 (NP_665805).

  • Positive control
    • Transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ZNF511 antibody images

  • Anti-ZNF511 antibody (ab87137) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cel lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : ~35 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF511 antibody (ab87137)

ab87137 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 22 2014

Thank you for contacting us. I am sorry to confirm that because we carry over 80,000 products, it is not feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up