The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTranscriptional regulator that plays a role in retinal development and maintenance.
Tissue specificityIn the retina, expressed in the outer and inner nuclear layers, and the ganglion cell layer.
Involvement in diseaseDefects in ZNF513 are the cause of retinitis pigmentosa type 58 (RP58) [MIM:613617]. A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family. Contains 8 C2H2-type zinc fingers.