For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- FunctionMay be involved in transcriptional regulation.
- Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 10 C2H2-type zinc fingers.
- Cellular localizationNucleus.
Information by UniProt
- C330029B10Rik antibody
- D230016L03 antibody
- HsT287 antibody
Anti-ZNF516 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF516 antibody (ab121486)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121486 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF516 antibody (ab121486)
ab121486, at 1/60 dilution, staining ZNF516 in paraffin-embedded Human prostate tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.
References for Anti-ZNF516 antibody (ab121486)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"