Overview

  • Product name
    Anti-ZNF516 antibody
  • Description
    Rabbit polyclonal to ZNF516
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 610-714 of Human ZNF516.

  • Positive control
    • Human prostate tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in transcriptional regulation.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 10 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C330029B10Rik antibody
    • D230016L03 antibody
    • HsT287 antibody
    • KIAA0222 antibody
    • RGD1306817 antibody
    • Zfp26l antibody
    • Zinc finger protein 516 antibody
    • ZN516_HUMAN antibody
    • Znf516 antibody
    see all

Anti-ZNF516 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121486 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121486, at 1/60 dilution, staining ZNF516 in paraffin-embedded Human prostate tissue by Immunohistochemistry, showing granular cytoplasmic positivity in glandular cells.

References for Anti-ZNF516 antibody (ab121486)

ab121486 has not yet been referenced specifically in any publications.

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Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (white adipose tissue, brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
80 µg
Specification
white adipose tissue, brown adipose tissue
Blocking step
Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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