Overview

  • Product nameAnti-ZNF540 antibody
    See all ZNF540 primary antibodies
  • Description
    Rabbit polyclonal to ZNF540
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 612 - 661 (CGKAFRLNSH LTEHQRIHTG EKPYECKVCR KAFRQYSHLY QHQKTHNVI) of human ZNF540 (NP_689819).

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceZNF540 belongs to the krueppel C2H2 type zinc finger protein family. It contains 17 C2H2 type zinc fingers and 1 KRAB domain and may be involved in transcriptional regulation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DKFZp313K2238 antibody
    • DKFZp547B0714 antibody
    • FLJ16004 antibody
    • Nbla10512 antibody
    • Putative protein product of Nbla10512 antibody
    • Zinc finger protein 540 antibody
    see all

Anti-ZNF540 antibody images

  • Anti-ZNF540 antibody (ab83420) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF540 antibody (ab83420)

ab83420 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"