Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF541
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: ASPSQVAMAS FSSAGPPADP SKSKLTIFSR IQGGNIYRLP HPVKEENVAG, corresponding to internal sequence amino acids 360-409 of Human ZNF541

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 87 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/1562500.

Target

Anti-ZNF541 antibody images

  • All lanes : Anti-ZNF541 antibody (ab66264) at 1.25 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 87 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF541 antibody (ab66264)

ab66264 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66264.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up