Overview

  • Product nameAnti-ZNF543 antibody
    See all ZNF543 primary antibodies
  • Description
    Rabbit polyclonal to ZNF543
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GCPLFKPELI YQLDHRQELW MATKDLSQSS YPGDNTKPKT TEPTFSHLAL PEEVLLQEQL TQGASKNSQL GQSK, corresponding to amino acids in Human ZNF543.

  • Positive control
    • Human rectum tissue; RT 4, U 251 MG, Human plasma, liver and tonsil lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 13 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434H055 antibody
    • Zinc finger protein 543 antibody
    • ZN543_HUMAN antibody
    • ZNF543 antibody

Anti-ZNF543 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122080 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122080, at 1/50 dilution, staining ZNF543 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-ZNF543 antibody (ab122080) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References for Anti-ZNF543 antibody (ab122080)

ab122080 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122080.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"