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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
- PurityImmunogen affinity purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
- FunctionMay be involved in transcriptional regulation.
- Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 13 C2H2-type zinc fingers.
Contains 1 KRAB domain.
- Cellular localizationNucleus.
Information by UniProt
- DKFZp434H055 antibody
- Zinc finger protein 543 antibody
- ZN543_HUMAN antibody
- ZNF543 antibody
Anti-ZNF543 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF543 antibody (ab122080)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122080 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF543 antibody (ab122080)
ab122080, at 1/50 dilution, staining ZNF543 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
Western blot - Anti-ZNF543 antibody (ab122080)
All lanes : Anti-ZNF543 antibody (ab122080) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
References for Anti-ZNF543 antibody (ab122080)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"