Overview

  • Product nameAnti-ZNF548 antibody
    See all ZNF548 primary antibodies
  • Description
    Rabbit polyclonal to ZNF548
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: HRVHMAEEIF TCMEGWKDLP ATSCLLQHQG PQSEWKPYRD TEDREAFQTG , corresponding to N terminal amino acids 144-193 of Human ZNF548

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

Target

Anti-ZNF548 antibody images

  • Anti-ZNF548 antibody (ab80426) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF548 antibody (ab80426)

ab80426 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"