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A region within synthetic peptide: PPPPKAPPFP KAEGPSSTPS SAAGPRPPRL GRHLLIDANG VPYTYTVQLE, corresponding to amino acids 21-70 of Human ZNF580
Our Abpromise guarantee covers the use of ab59015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ZNF580 with ab59015 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the fundic gland. Magnification: 400X.
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