Overview

  • Product nameAnti-ZNF589 antibody
    See all ZNF589 primary antibodies
  • Description
    Rabbit polyclonal to ZNF589
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 39-183 of Human ZNF589 (Q86UQ0).

  • Positive control
    • RT4 and U-251 MG cell lysates; Human bone marrow tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in hematopoietic stem/progenitor cell differentiation. May play a role as a DNA binding-dependent transcriptional repressor.
  • Tissue specificityIsoform 2 is widely expressed. Isoform 3 is only expressed in CD34(+) cells.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • DomainThe KRAB domain mediates interaction with TRIM28.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KRAB zinc finger protein SZF1 1 antibody
    • Stem cell zinc finger protein 1 antibody
    • SZF1 antibody
    • Zinc finger protein 589 antibody
    • ZN589_HUMAN antibody
    • ZNF589 antibody
    • ZNP589 antibody
    see all

Anti-ZNF589 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei and cytoplasm. Recommended concentration of ab121173 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ZNF589 antibody (ab121173) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121173 at 1/100 dilution staining ZNF589 in Paraffin-emebedded Human bone marrow tissue by Immunohistochemistry.

References for Anti-ZNF589 antibody (ab121173)

ab121173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"