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Rabbit polyclonal to ZNF589
Recombinant fragment, corresponding to amino acids 39-183 of Human ZNF589 (Q86UQ0).
- RT4 and U-251 MG cell lysates; Human bone marrow tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in hematopoietic stem/progenitor cell differentiation. May play a role as a DNA binding-dependent transcriptional repressor.
Isoform 2 is widely expressed. Isoform 3 is only expressed in CD34(+) cells.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Contains 1 KRAB domain.
The KRAB domain mediates interaction with TRIM28.
Information by UniProt
- KRAB zinc finger protein SZF1 1 antibody
- Stem cell zinc finger protein 1 antibody
- SZF1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ZNF589 antibody (ab121173)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei and cytoplasm. Recommended concentration of ab121173 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-ZNF589 antibody (ab121173)
All lanes : Anti-ZNF589 antibody (ab121173) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF589 antibody (ab121173)
ab121173 at 1/100 dilution staining ZNF589 in Paraffin-emebedded Human bone marrow tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"