Overview

  • Product nameAnti-ZNF589 antibody
    See all ZNF589 primary antibodies
  • Description
    Rabbit polyclonal to ZNF589
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: NRILEIQLSP AQNASSEEVD RISKRAETPG FGAVTFGECA LAFNQKSNLF, corresponding to internal sequence amino acids 179-228 of Human ZNF589

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab49299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

Target

  • FunctionMay play a role in hematopoietic stem/progenitor cell differentiation. May play a role as a DNA binding-dependent transcriptional repressor.
  • Tissue specificityIsoform 2 is widely expressed. Isoform 3 is only expressed in CD34(+) cells.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • DomainThe KRAB domain mediates interaction with TRIM28.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KRAB zinc finger protein SZF1 1 antibody
    • Stem cell zinc finger protein 1 antibody
    • SZF1 antibody
    • Zinc finger protein 589 antibody
    • ZN589_HUMAN antibody
    • ZNF589 antibody
    • ZNP589 antibody
    see all

Anti-ZNF589 antibody images

  • Anti-ZNF589 antibody (ab49299) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF589 antibody (ab49299)

ab49299 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49299.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"