Overview

  • Product nameAnti-ZNF621 antibody
    See all ZNF621 primary antibodies
  • Description
    Rabbit polyclonal to ZNF621
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide: LSSNTALTQH QRIHTGEKPY ECKECGKAFR RSAAYLQHQR LHTGEKLYKC, corresponding to a region within internal sequence amino acids 216-265 of Human ZNF621

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686B11107 antibody
    • FLJ23786 antibody
    • FLJ45246 antibody
    • MGC126668 antibody
    • Zinc finger protein 621 antibody
    • ZN621_HUMAN antibody
    • ZNF621 antibody
    see all

Anti-ZNF621 antibody images

  • Anti-ZNF621 antibody (ab49225) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF621 antibody (ab49225)

ab49225 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"