Anti-ZNF653 antibody (ab121769)
Key features and details
- Rabbit polyclonal to ZNF653
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNF653 antibody -
Description
Rabbit polyclonal to ZNF653 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: NVNCLKNVVI WYEDHKHRCP YEPHLAELDP TFGLYTTAVW QCEAGHRYFQ DLHSPLKPLS DSDPDSDKVG NGLVAGSSDS SSSGS, corresponding to amino acids 119-203 of Human ZNF653.
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Positive control
- IHC: Human cerebral cortex tissue WB: RT 4 and U 251 MG lysate ICC/IF: human cell line U-2 OS
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Transcriptional repressor. May repress NR5A1, PPARG, NR1H3, NR4A2, ESR1 and NR3C1 transcriptional activity. -
Tissue specificity
Highly expressed in testis, cerebellum, temporal lobe, hippocampus and the adrenal gland. Moderately expressed in spleen, uterus, thymus, pancreas, kidney, stomach and rectum. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 115950 Human
- Omim: 611371 Human
- SwissProt: Q96CK0 Human
- Unigene: 465928 Human
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Alternative names
- 67 kDa zinc finger protein antibody
- Zinc finger protein 653 antibody
- Zinc finger protein Zip67 antibody
see all
Images
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Immunocytochemistry/immunofluorescence analysis of human cell line U-2 OS labelling ZNF653 with ab121769 at 2 µg/mL or dilution
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ab121769 at 1/50 dilution staining ZNF653 in paraffin embedded Human cerebral cortex tissue by Immunohistochemistry.
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All lanes : Anti-ZNF653 antibody (ab121769) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121769 has not yet been referenced specifically in any publications.