Overview

  • Product nameAnti-ZNF675 antibody
    See all ZNF675 primary antibodies
  • Description
    Rabbit polyclonal to ZNF675
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: AFNRSSKLTE YKKLHTGKKP YKCEECGKAF IQSSKLTEHK KIHSGEIPYK, corresponding to C terminal amino acids 432-481 of Human ZNF675

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab69899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-ZNF675 antibody images

  • Anti-ZNF675 antibody (ab69899) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 61 kDa (why is the actual band size different from the predicted?)

References for Anti-ZNF675 antibody (ab69899)

ab69899 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"