Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNF704
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: TIPGSAKFTP NGSSFSISWQ SPPVTFTGIP VSPTHHPVGT GEQRQHAHTV LSSPPRGTVS LRKPRGEGKK CRKVYGMENR DMWCT, corresponding to amino acids 315-399 of Human ZNF704 (Q6ZNC4).

  • Positive control
    • Human small intestine tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121804 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-ZNF704 antibody (ab121804)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing ZNF704 with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemical staining of paraffin embedded Human small intestine tissue using 1/50 ab121804.

References

ab121804 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121804.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up