Overview

  • Product name
    Anti-ZNF71 antibody
  • Description
    Rabbit polyclonal to ZNF71
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 440-489 (CGQCGKSFIK NSSLTVHQRI HTGEKPYRCG ECGKTFSRNT NLTRHLRIHT) of Human ZNF71 (NP_067039).

  • Positive control
    • PANC1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    May be involved in transcriptional regulation.
  • Tissue specificity
    Highly expressed in placenta, followed by brain, testis, pancreas, heart, small intestine, muscle, uterus, prostate and peripheral blood leukocytes. Not detected in liver, lung, colon, stomach, salivary and thyroid gland.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 13 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cos26 antibody
    • Endothelial zinc finger protein antibody
    • Endothelial zinc finger protein induced by tumor necrosis factor alpha antibody
    • EZFIT antibody
    • Kruppel related zinc finger protein antibody
    • Zinc finger protein 71 (Cos26) antibody
    • Zinc finger protein 71 antibody
    • ZNF47 antibody
    • ZNF71 antibody
    • ZNF71_HUMAN antibody
    see all

Images

  • Anti-ZNF71 antibody (ab87250) at 1 µg/ml (in 5% skim milk / PBS buffer) + PANC1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • ab87250 at 5 µg/ml staining ZNF71 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab87250 at 5 µg/ml staining ZNF71 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab87250 at 5 µg/ml staining ZNF71 in Human uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab87250 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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