Overview

  • Product nameAnti-ZNF8 antibody
    See all ZNF8 primary antibodies
  • Description
    Rabbit polyclonal to ZNF8
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 525 and 575 of Human ZNF8 (NP_066575.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 65 kDa.
IP Use at 5-10 µg/mg of lysate.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Tissue specificityUbiquitously present in many human cell lines of different embryological derivation.
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HF.18 antibody
    • Zfp128 antibody
    • Zinc finger protein 272 antibody
    • Zinc finger protein 8 (clone HF.18) antibody
    • Zinc finger protein 8 antibody
    • Zinc finger protein HF.18 antibody
    • ZNF 8 antibody
    • ZNF8 antibody
    • ZNF8_HUMAN antibody
    see all

Anti-ZNF8 antibody images

  • All lanes : Anti-ZNF8 antibody (ab87732) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 65 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of ZNF8 in Immunoprecipitates of Hela whole cell lysate (1 mg for IP, 20% of IP loaded) using ab87732 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-ZNF8 antibody (ab87732)

ab87732 has not yet been referenced specifically in any publications.

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