Overview

  • Product nameAnti-ZNF81 antibody
    See all ZNF81 primary antibodies
  • Description
    Rabbit polyclonal to ZNF81
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: GKAFTNRSNL NTHQKSHTGE KSYICAECGK AFTDRSNFNK HQTIHTGEKP, corresponding to a region within internal sequence amino acids 503-553 of Human ZNF81

  • Positive control
    • Jurkat cell lysats Human liver tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.
IHC-P Use a concentration of 4 - 8 µg/ml.
IP Use at an assay dependent concentration.

Target

  • FunctionMay be involved in transcriptional regulation.
  • Involvement in diseaseDefects in ZNF81 are the cause of mental retardation X-linked type 45 (MRX45) [MIM:300498]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Note=A chromosomal aberration involving ZNF81 is found in a severe mental retardation patient. Translocation t(X;9)(p11.23;q34.3).
  • Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 12 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HFZ20 antibody
    • Zinc finger protein 81 antibody
    • ZNF81 antibody
    • ZNF81_HUMAN antibody

Anti-ZNF81 antibody images

  • Anti-ZNF81 antibody (ab49119) at 2 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • Human liver tissue with positively stained hepatocytes (arrows). Antibody concentration 4-8µg/ml. Magnification: 400x

References for Anti-ZNF81 antibody (ab49119)

ab49119 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (K562 nuclear extract)
Total protein in input 25 µg
Specification K562 nuclear extract
Immuno-precipitation step Protein A/G
Username

Dr. Seth Frietze

Verified customer

Submitted Oct 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"