Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab154640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-ZNF83 antibody - N-terminal (ab154640) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling ZNF38 with ab154640 at a 1/1000 dilution.
    The lower image has been stained with Hoechst 44432.
  • Immunohistochemical analysis of paraffin embedded Huh-7 xenograft labeling ZNF83 with ab154640 at a 1/500 dilution.

References

ab154640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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