The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionDNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
Tissue specificityHighly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
Sequence similaritiesBelongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers.
DomainThe N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members. C-terminal zinc fingers mediate homodimerization.