Recombinant
RabMAb

Anti-ZNFN1A4 antibody [EPR11804] (ab170957)

Overview

  • Product name
    Anti-ZNFN1A4 antibody [EPR11804]
    See all ZNFN1A4 primary antibodies
  • Description
    Rabbit monoclonal [EPR11804] to ZNFN1A4
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ZNFN1A4. The exact sequence is proprietary.
    Database link: Q9H2S9

  • Positive control
    • HeLa, HepG2, THP1 and Human skeletal muscle lysates; HeLa and THP1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11804
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 64 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems.
    • Tissue specificity
      Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.
    • Sequence similarities
      Belongs to the Ikaros C2H2-type zinc-finger protein family.
      Contains 6 C2H2-type zinc fingers.
    • Domain
      The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
      C-terminal zinc fingers mediate homodimerization.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • EOS antibody
      • Eos protein antibody
      • IKAROS family zinc finger 4 (Eos) antibody
      • IKAROS family zinc finger 4 antibody
      • Ikaros family zinc finger protein 4 antibody
      • IKZF4 antibody
      • IKZF4_HUMAN antibody
      • KIAA1782 antibody
      • Zinc finger protein Eos (IKAROS family zinc finger protein 4) antibody
      • Zinc finger protein Eos antibody
      • Zinc finger protein, subfamily 1A, 4 (Eos) antibody
      • Zinc finger protein, subfamily 1A, member 4 antibody
      • Zinc finger transcription factor Eos antibody
      • ZNFN1A4 antibody
      see all

    Images

    • Western blot analysis on Immunoprecipitation pellet from HeLa cell lysate showing ZNFN1A4 , using ab170957 at 1/10 dilution for IP.

    • All lanes : Anti-ZNFN1A4 antibody [EPR11804] (ab170957) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Human skeletal lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 64 kDa

    References

    ab170957 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170957.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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