Anti-ZNHIT1 antibody (ab151248)
Key features and details
- Rabbit polyclonal to ZNHIT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZNHIT1 antibody
See all ZNHIT1 primary antibodies -
Description
Rabbit polyclonal to ZNHIT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein corresponding to amino acids 1-154 of Human ZNHIT1 (UniProt ID: O43257).
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Positive control
- MOLT-4 whole cell lysate; Raji cells; HeLa cells; SW480 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
ZNHIT1, Zinc finger HIT domain containing protein 1, appears to play a role in p53-mediated apoptosis induction. -
Database links
- Entrez Gene: 514997 Cow
- Entrez Gene: 10467 Human
- Entrez Gene: 70103 Mouse
- SwissProt: Q24JY4 Cow
- SwissProt: O43257 Human
- SwissProt: Q8R331 Mouse
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Alternative names
- CG1I antibody
- CGBP1 antibody
- Cyclin G1 binding protein 1 antibody
see all
Images
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Anti-ZNHIT1 antibody (ab151248) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ZNHIT1 with ab151248 at 1/100 dilution. Lower panel was merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded SW480 xenograft tissue labeling ZNHIT1 with ab151248 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151248 has not yet been referenced specifically in any publications.