Anti-ZNHIT2 antibody (ab118133)

Overview

  • Product nameAnti-ZNHIT2 antibody
    See all ZNHIT2 primary antibodies
  • Description
    Mouse monoclonal to ZNHIT2
  • Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 274-362 of Human ZNHIT2 with proprietary tag (NP_055020).

Properties

Applications

Our Abpromise guarantee covers the use of ab118133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • Tissue specificityLow expression in most tissues; highly expressed in testis.
  • Sequence similaritiesContains 1 HIT-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf5 antibody
    • FON antibody
    • MGC120285 antibody
    • MGC120286 antibody
    • OTTHUMP00000230419 antibody
    • Protein FON antibody
    • Zinc finger HIT domain-containing protein 2 antibody
    • Zinc finger HIT type containing 2 antibody
    • zinc finger, HIT domain containing 2 antibody
    • zinc finger, HIT type 2 antibody
    • ZNHI2_HUMAN antibody
    • ZNHIT2 antibody
    see all

Anti-ZNHIT2 antibody images

  • Detection limit for recombinant tagged ZNHIT2 is 1 ng/ml as a capture antibody.

References for Anti-ZNHIT2 antibody (ab118133)

ab118133 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview for ab118133. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested ssamples and application, we would like to follo...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa S3)
Loading amount 5.6 µg
Specification HeLa S3
Treatment two succesive rounds of siRNA (100 nM). One directed agaist ZNHIT2 and three controls
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 30 2012

Thank you for the image. I encourage you to include the image in your review. I agree it does not look very good but this is useful data, insofar as it shows what does not work.

The discount code will be active as soon as you submit your rev...

Read More

I am very pleased to hear you would like to accept our offer and test ab118133 in western blotting. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for western blotting and i...

Read More

Thank you for your reply, yes, we would be interested in adding western blot data. As you can see from the datasheet, the antibody is not very well-characterized, and specificity has not been tested on endogenous samples of any kind. What kind of sampl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"