Overview

  • Product name
  • Description
    Rabbit polyclonal to ZNRF2
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human ZNRF2 (NP_667339.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114098 was affinity purified using an epitope specific to ZNRF2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
    • Tissue specificity
      Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 RING-type zinc finger.
    • Domain
      The RING-type zinc finger domain is required for E3 ligase activity.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Endosome membrane. Lysosome membrane. Cell junction > synapse > presynaptic cell membrane. Present in presynaptic plasma membranes in neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 ubiquitin-protein ligase ZNRF2 antibody
      • Protein Ells2 antibody
      • RING finger protein 202 antibody
      • RNF202 antibody
      • Zinc/RING finger protein 2 antibody
      • Znrf2 antibody
      • ZNRF2_HUMAN antibody
      see all

    Images

    • Rabbit anti-ZNRF2 antibody, at 1 µg/ml, staining ZNRF2 in HeLa whole cell lysate immunoprecipitated using ab114098 at 10 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab114098 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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