The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionMay play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Tissue specificityHighly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
PathwayProtein modification; protein ubiquitination.
Rabbit anti-ZNRF2 antibody, at 1 µg/ml, staining ZNRF2 in HeLa whole cell lysate immunoprecipitated using ab114098 at 10 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-ZNRF2 antibody (ab114098)
has not yet been referenced specifically in any publications.
Publishing research using ab114098? Please let us know so that we can cite the reference in this datasheet.
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