Overview

  • Product name
    Anti-ZNRF2 antibody [EPR11399(B)]
    See all ZNRF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11399(B)] to ZNRF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ZNRF2. The exact sequence is proprietary.

  • Positive control
    • Jurkat, HeLa, Human fetal brain and Neuro-2a lysates, Human ovarian carcinoma tissue, Human testis tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
    • Tissue specificity
      Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 RING-type zinc finger.
    • Domain
      The RING-type zinc finger domain is required for E3 ligase activity.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Endosome membrane. Lysosome membrane. Cell junction > synapse > presynaptic cell membrane. Present in presynaptic plasma membranes in neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 ubiquitin-protein ligase ZNRF2 antibody
      • Protein Ells2 antibody
      • RING finger protein 202 antibody
      • RNF202 antibody
      • Zinc/RING finger protein 2 antibody
      • Znrf2 antibody
      • ZNRF2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ZNRF2 antibody [EPR11399(B)] (ab156011) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : HeLa lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Neuro-2a lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 24 kDa

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.

    References

    This product has been referenced in:
    • Lee CY  et al. The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation. Nat Commun 8:15502 (2017). Read more (PubMed: 28593998) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab156011.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up