Anti-ZO1 tight junction protein antibody (ab96587)


  • Product nameAnti-ZO1 tight junction protein antibody
    See all ZO1 tight junction protein primary antibodies
  • Description
    Rabbit polyclonal to ZO1 tight junction protein
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.

  • Positive control
    • 293T, A431, H1299 or HeLaS3 cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab96587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 187 kDa.


  • FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • DomainThe second PDZ domain mediates interaction with GJA12.
  • Post-translational
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody images

  • Anti-ZO1 tight junction protein antibody (ab96587) at 1/3000 dilution (5% SDS PAGE) + H1299 whole cell lysate at 30 µg

    Predicted band size : 187 kDa
  • Immunohistochemical analysis of paraffin embedded mouse intestine tissue labeling ZO1 with ab96587 at 1:500 dilution.

  • Immunohistochemical analysis of paraffin embedded mouse testis labeling ZO1 with ab96587 at 1:500 dilution.

  • Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + Mouse testis tissue extract at 50 µg

    HRP conjugated anti-rabbit IgG

    Predicted band size : 187 kDa

References for Anti-ZO1 tight junction protein antibody (ab96587)

ab96587 has not yet been referenced specifically in any publications.

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Unfortunately ab96587 has not been tested in any applications other than WB. It doesn’t mean it won’t work in IHC, but we don’t have data to support it.

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