Anti-ZO1 tight junction protein antibody (ab96587)
Key features and details
- Rabbit polyclonal to ZO1 tight junction protein
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ZO1 tight junction protein antibody
See all ZO1 tight junction protein primary antibodies -
Description
Rabbit polyclonal to ZO1 tight junction protein -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.
-
Positive control
- WB: HEK-293T, HeLa, Neuro-2a, C8-D30, NIH/3T3 and C2C12 cell lysate, mouse testis tissue lysate. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells and H661 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/1000.
Fix cells in ice-cold MeOH |
|
WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 187 kDa.
5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.
Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
Notes |
---|
ICC/IF
1/100 - 1/1000. Fix cells in ice-cold MeOH |
WB
1/500 - 1/3000. Predicted molecular weight: 187 kDa. 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.
Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
Target
-
Function
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. -
Tissue specificity
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. -
Domain
The second PDZ domain mediates interaction with GJA12. -
Post-translational
modificationsPhosphorylated. Dephosphorylated by PTPRJ. -
Cellular localization
Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact. - Information by UniProt
-
Database links
- Entrez Gene: 7082 Human
- Entrez Gene: 21872 Mouse
- Omim: 601009 Human
- SwissProt: Q07157 Human
- SwissProt: P39447 Mouse
- Unigene: 510833 Human
- Unigene: 4342 Mouse
-
Alternative names
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
see all
Images
-
All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition:Semi-dry, 18 V, 60min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL detection.
-
Immunofluorescence analysis of ice-cold methanol-fixed (5 min) NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells labeling ZO1 tight junction protein at cell membrane (green) with ab96587 at 1/200 dilution. Blue: Hoechst 33342 staining.
-
All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
-
All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : ZO1 tight junction protein shRNA-transfected HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 187 kDa5% SDS-PAGE
-
Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + Mouse testis tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
-
Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (152)
ab96587 has been referenced in 152 publications.
- Satitsri S et al. Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model. BMC Vet Res 19:4 (2023). PubMed: 36624444
- Nakamura A et al. The potential of soluble CD14 in discriminating nonalcoholic steatohepatitis from nonalcoholic fatty liver disease. Hepatol Res 52:508-521 (2022). PubMed: 35129841
- Nyandwi JB et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
- Xiong Y et al. Sodium Butyrate Attenuates Taurocholate-Induced Acute Pancreatitis by Maintaining Colonic Barrier and Regulating Gut Microorganisms in Mice. Front Physiol 13:813735 (2022). PubMed: 35370779
- Li DK et al. Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis. Cancer Gene Ther 29:1439-1451 (2022). PubMed: 35388172