Overview

  • Product nameAnti-ZO1 tight junction protein antibody
    See all ZO1 tight junction protein primary antibodies
  • Description
    Goat polyclonal to ZO1 tight junction protein
  • Specificityab99462 is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2).
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Peptide with sequence

    C-PKPTSQNQFSEHDKT

    , from the internal region of the protein sequence according to NP_003248.3; NP_783297.2.

  • Positive control
    • Human brain (cerebral cortex) lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 195 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 24874215

Target

  • FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similaritiesBelongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • DomainThe second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Anti-ZO1 tight junction protein antibody images

  • Anti-ZO1 tight junction protein antibody (ab99462) at 0.3 µg/ml + Human brain (cerebral cortex) lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 195 kDa


    Exposure time : 1 hour

References for Anti-ZO1 tight junction protein antibody (ab99462)

This product has been referenced in:
  • Lundin A  et al. Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog 10:e1004166 (2014). ICC/IF ; Human . Read more (PubMed: 24874215) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"