The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 195 kDa.
Use at an assay dependent concentration. PubMed: 24874215
FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Sequence similaritiesBelongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. Contains 1 ZU5 domain.
DomainThe second PDZ domain mediates interaction with GJA12.
Post-translational modificationsPhosphorylated. Dephosphorylated by PTPRJ.
Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
Western blot - ZO1 tight junction protein antibody (ab99462)
Anti-ZO1 tight junction protein antibody (ab99462) at 0.3 µg/ml + Human brain (cerebral cortex) lysate at 35 µg Developed using the ECL technique
Predicted band size : 195 kDa
Exposure time : 1 hour
References for Anti-ZO1 tight junction protein antibody (ab99462)
This product has been referenced in:
Lundin A et al. Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog10:e1004166 (2014).
Read more (PubMed: 24874215) »