Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)

Overview

  • Product name
    Anti-ZO1 tight junction protein antibody - C-terminal
    See all ZO1 tight junction protein primary antibodies
  • Description
    Goat polyclonal to ZO1 tight junction protein - C-terminal
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Monkey
  • Immunogen

    Recombinant fragment within Human ZO1 tight junction protein aa 1580-1748 (C terminal). The exact sequence is proprietary. (Purified from E.coli; NP_003248.3).
    Database link: Q07157

  • Positive control
    • Human kidney tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/250.
WB 1/500 - 1/2000. Predicted molecular weight: 195 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
  • Tissue specificity
    The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
  • Sequence similarities
    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
    Contains 1 ZU5 domain.
  • Domain
    The second PDZ domain mediates interaction with GJA12.
  • Post-translational
    modifications
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Cellular localization
    Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tight junction protein 1 antibody
    • Tight junction protein ZO-1 antibody
    • Tight junction protein ZO1 antibody
    • TJP1 antibody
    • zo-1 antibody
    • Zo1 antibody
    • ZO1_HUMAN antibody
    • Zona occludens 1 antibody
    • Zona occludens 1 protein antibody
    • Zona occludens protein 1 antibody
    • Zonula occludens 1 protein antibody
    • Zonula occludens protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 µg/ml.

References

This product has been referenced in:
  • Hosoya M  et al. Overlapping expression of anion exchangers in the cochlea of a non-human primate suggests functional compensation. Neurosci Res 110:1-10 (2016). Read more (PubMed: 27091614) »
  • Hosoya M  et al. Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea. Sci Rep 6:22250 (2016). Read more (PubMed: 26915689) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Colonic epithelial cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Colonic epithelial cells
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Methanol
Username

mahesha yatigalpotht

Verified customer

Submitted Nov 20 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
Yes - 0.5% Triton-X 100
Specification
small intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (Oviduct, epithelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Permeabilization
No
Specification
Oviduct, epithelial cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Primary tracheal epithelial cell)
Permeabilization
Yes - 0.5% Triton X-100
Specification
Primary tracheal epithelial cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Nicky O'Boyle

Verified customer

Submitted Jan 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Embryo (Mouse embryo tissue)
Specification
Mouse embryo tissue
Username

Patricia Ybot

Verified customer

Submitted Oct 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Rabbit cornea. Limbal area.)
Permeabilization
No
Specification
Rabbit cornea. Limbal area.
Blocking step
5% BSA + 10% FBS in PBT (PBS + 0,1% triton) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Noelia Andollo

Verified customer

Submitted Jul 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (neurulation stage chick embryos)
Permeabilization
Yes - 0.3% tritonX-100 in PBS
Specification
neurulation stage chick embryos
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
10% trichloroacetic acid in PBS
Username

Ms. Amanda Baumholtz

Verified customer

Submitted Oct 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up