Overview

  • Product nameAnti-zona radiata antibody
    See all zona radiata primary antibodies
  • Description
    Rabbit polyclonal to zona radiata
  • SpecificityCross reactivity with zona pellucida proteins may be observed in fish ovaries or eggs.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rainbow Trout
  • Immunogen

    Eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) purified from plasma of 17 beta estradiol treated Atlantic salmon

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/50 - 1/2000 (Salmoniformes). As the antibody is dissolved in a buffer containing BSA it is not recommended to use for coating.

    WB: 1/50 - 1/2000 (Salmoniformes). Predicted molecular weight: 45 kDa.

    Note: for both applications 1/50 - 1/200 working dilutions are recommended when dealing with samples from other species.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceZona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
    • Database links
      • Alternative names
        • ZRP antibody

      Anti-zona radiata antibody images

      • Coating with plasma (diluted 1:1000) from 17 beta-estradiol treated (E2) or control (C) fish.
      • All lanes : Anti-zona radiata antibody (ab36803) at 1/100 dilution

        Lane 2 : Plasma from control Salmon
        Lane 3 : Plasma from 17ß-estradiol treated Salmon
        Lane 4 : Plasma from control Rainbow Trout
        Lane 5 : Plasma from 17ß-estradiol treated Rainbow trout
        Lane 6 : Plasma from control Arctic char
        Lane 7 : Plasma from 17ß-estradiol treated Arctic char
        Lane 8 : Plasma from control Atlantic cod
        Lane 9 : Plasma from 17ß-estradiol treated Atlantic cod
        Lane 10 : Plasma from control Turbot
        Lane 11 : Plasma from 17ß-estradiol treated Turbot
        Lane 12 : Plasma from control Flounder
        Lane 13 : Plasma from 17ß-estradiol treated Flounder
        Lane 14 : Plasma from control Arctic char
        Lane 15 : Plasma from 17ß-estradiol treated Arctic char


        Predicted band size : 45 kDa

      References for Anti-zona radiata antibody (ab36803)

      ab36803 has not yet been referenced specifically in any publications.

      Product Wall

      There are currently no Abreviews or Questions for ab36803.
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"