Note: for both applications 1/50 - 1/200 working dilutions are recommended when dealing with samples from other species.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceZona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
Coating with plasma (diluted 1:1000) from 17 beta-estradiol treated (E2) or control (C) fish.
Western blot - zona radiata antibody (ab36803)
All lanes : Anti-zona radiata antibody (ab36803) at 1/100 dilution
Lane 2 : Plasma from control Salmon Lane 3 : Plasma from 17ß-estradiol treated Salmon Lane 4 : Plasma from control Rainbow Trout Lane 5 : Plasma from 17ß-estradiol treated Rainbow trout Lane 6 : Plasma from control Arctic char Lane 7 : Plasma from 17ß-estradiol treated Arctic char Lane 8 : Plasma from control Atlantic cod Lane 9 : Plasma from 17ß-estradiol treated Atlantic cod Lane 10 : Plasma from control Turbot Lane 11 : Plasma from 17ß-estradiol treated Turbot Lane 12 : Plasma from control Flounder Lane 13 : Plasma from 17ß-estradiol treated Flounder Lane 14 : Plasma from control Arctic char Lane 15 : Plasma from 17ß-estradiol treated Arctic char
Predicted band size : 45 kDa
References for Anti-zona radiata antibody (ab36803)
has not yet been referenced specifically in any publications.
Publishing research using ab36803? Please let us know so that we can cite the reference in this datasheet.
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