Anti-zona radiata antibody [MN-7F2] (ab36802)

Overview

  • Product name
    Anti-zona radiata antibody [MN-7F2]
    See all zona radiata primary antibodies
  • Description
    Mouse monoclonal [MN-7F2] to zona radiata
  • Specificity
    This antibody binds with high affinity to zona radiata proteins (Zrp) in plasma samples from Atlantic salmon (Salmo salar), reacting primarily with the g monomer.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rainbow trout
  • Immunogen

    Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).

  • Positive control
    • Plasma from 17ß-estradiol treated Salmon.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    MN-7F2
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/100 - 1/5000.
    WB: 1/100 - 1/1000. Detects a band of approximately 45 kDa.
    The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Zona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
    • Database links
      • Alternative names
        • ZRP antibody

      Images

      • Coating: Plasma (diluted 1:1000) from 17ß-estradiol treated (E2) or control (C) fish.
      • Lane 1 : Molecular weight standards
        Lanes 2 - 10 : Anti-zona radiata antibody [MN-7F2] (ab36802) at 1/500 dilution

        Lane 1 : As above
        Lane 2 : Purified Zrp, [alpha, beta and gamma].
        Lane 3 : Plasma from 17ß-estradiol treated Salmon at 1/200 dilution
        Lane 4 : Plasma from control Salmon at 1/200 dilution
        Lane 5 : Plasma from 17ß-estradiol treated Arctic char at 1/200 dilution
        Lane 6 : Plasma from control Arctic char at 1/200 dilution
        Lane 7 : Plasma from 17ß-estradiol treated Turbot at 1/200 dilution
        Lane 8 : Plasma from control Turbot at 1/200 dilution
        Lane 9 : Plasma from 17ß-estradiol treated Cod at 1/200 dilution
        Lane 10 : Plasma from control Cod at 1/200 dilution

      References

      ab36802 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      There are currently no Customer reviews or Questions for ab36802.
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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