Anti-zona radiata antibody [MN-8C4] (ab36883)

Overview

  • Product nameAnti-zona radiata antibody [MN-8C4]
    See all zona radiata primary antibodies
  • Description
    Mouse monoclonal [MN-8C4] to zona radiata
  • SpecificityAb36883 binds with high affinity to zona radiata proteins, reacting primarily with the alpha and beta monomers.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rainbow Trout
  • Immunogen

    Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from Atlantic salmon (Salmo salar).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMN-8C4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/100 - 1/5000. The antibody is dissolved in a buffer containing BSA and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceZona radiata is a thickened, rather complex egg membrane of teleost fishes, which often has a radiate appearance. It is formed at the surface of the egg by the ooplasm, or the ooplasm and the follicle cells and hence should be regarded as a true vitelline membrane. May be overlain by the chorion, the zona radiata underlies or overlies a homogeneous vitelline egg membrane.
    • Database links
      • Alternative names
        • ZRP antibody

      Anti-zona radiata antibody [MN-8C4] images

      • Ab36883, staining zona radiata by ELISA. Coating with plasma (diluted 1/1000) from 17 beta estradiol treated (E2) or control (C) fish.


      • Predicted band size : 46 kDa
        Observed band size : 46 kDa
        Additional bands at : 50 kDa (possible multimer),55 kDa (possible multimer).

      References for Anti-zona radiata antibody [MN-8C4] (ab36883)

      ab36883 has not yet been referenced specifically in any publications.

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      There are currently no Abreviews or Questions for ab36883.
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"