Overview

  • Product name
  • Description
    Rabbit polyclonal to ZP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide designed within residues: VGFEDSYGQE PTLGPTDSNG NSSLRPLLWA VLLLPAVALV LGFGVFVGLS, corresponding to internal amino acids 576 - 625 of Human ZP1 (NP_997224).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
  • Tissue specificity
    Oocytes.
  • Sequence similarities
    Belongs to the ZP domain family. ZPB subfamily.
    Contains 1 P-type (trefoil) domain.
    Contains 1 ZP domain.
  • Domain
    The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • Post-translational
    modifications
    Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    O-glycosylated.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC87693 antibody
    • Processed zona pellucida sperm-binding protein 1 antibody
    • zona pellucida glycoprotein 1 (sperm receptor) antibody
    • Zona pellucida glycoprotein 1 antibody
    • zona pellucida protein 1 antibody
    • Zp 1 antibody
    • Zp-1 antibody
    • Zp1 antibody
    • ZP1_HUMAN antibody
    see all

Anti-ZP1 antibody images

  • Anti-ZP1 antibody (ab90480) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa

References for Anti-ZP1 antibody (ab90480)

ab90480 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting my colleague at Abcam. To our knowledge, no one has tested this for ICC/IF. Trying a different fixative, for instance methanol instead of paraformaldehyde, may give a better result, but we have not tried either.

I r...

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