Overview

  • Product name
    Anti-ZP1 antibody [EPR10827]
    See all ZP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10827] to ZP1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZP1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: P60852

  • Positive control
    • NIH: OVCAR-3, SK-OV-3, LOVO cell line lysates and Human ovary cancer tissue lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab171954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
    • Tissue specificity
      Oocytes.
    • Sequence similarities
      Belongs to the ZP domain family. ZPB subfamily.
      Contains 1 P-type (trefoil) domain.
      Contains 1 ZP domain.
    • Domain
      The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
    • Post-translational
      modifications
      Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
      O-glycosylated.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC87693 antibody
      • Processed zona pellucida sperm-binding protein 1 antibody
      • zona pellucida glycoprotein 1 (sperm receptor) antibody
      • Zona pellucida glycoprotein 1 antibody
      • zona pellucida protein 1 antibody
      • Zp 1 antibody
      • Zp-1 antibody
      • Zp1 antibody
      • ZP1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ZP1 antibody [EPR10827] (ab171954) at 1/1000 dilution

      Lane 1 : NIH: OVCAR-3 cell lysate
      Lane 2 : SK-OV-3 cell lysate
      Lane 3 : LOVO cell lysate
      Lane 4 : Human ovary cancer tissue lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 70 kDa

    References

    This product has been referenced in:
    • Hu X  et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). Read more (PubMed: 25760093) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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