Recombinant Anti-ZP1 antibody [EPR10827] (ab171954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10827] to ZP1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ZP1 antibody [EPR10827]
See all ZP1 primary antibodies -
Description
Rabbit monoclonal [EPR10827] to ZP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ZP1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: P60852 -
Positive control
- NIH: OVCAR-3, SK-OV-3, LOVO cell line lysates and Human ovary cancer tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10827 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa. |
Target
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Function
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida. -
Tissue specificity
Oocytes. -
Sequence similarities
Belongs to the ZP domain family. ZPB subfamily.
Contains 1 P-type (trefoil) domain.
Contains 1 ZP domain. -
Domain
The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida. -
Post-translational
modificationsProteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
O-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 22917 Human
- Omim: 195000 Human
- SwissProt: P60852 Human
- Unigene: 172130 Human
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Alternative names
- MGC87693 antibody
- Processed zona pellucida sperm-binding protein 1 antibody
- zona pellucida glycoprotein 1 (sperm receptor) antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab171954 has been referenced in 1 publication.
- Hu X et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). PubMed: 25760093