Overview

  • Product nameAnti-ZP2 antibody
    See all ZP2 primary antibodies
  • Description
    Mouse monoclonal to ZP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 400-510 of Human ZP2 with a Proprietary tag (NP_003451).

  • Positive control
    • Recombinant fragment of ZP2 protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionThe mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
  • Tissue specificityOocytes.
  • Sequence similaritiesBelongs to the ZP domain family. ZPA subfamily.
    Contains 1 ZP domain.
  • Developmental stageExpressed during the 2-week growth phase of oogenesis, prior to ovulation.
  • DomainThe ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • Post-translational
    modifications
    Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
    O-glycosylated; contains sulfate-substituted glycans.
  • Cellular localizationSecreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000115849 antibody
    • Processed zona pellucida sperm-binding protein 2 antibody
    • Zona pellucida glycoprotein 2 antibody
    • zona pellucida glycoprotein ZP2 antibody
    • Zona pellucida protein A antibody
    • Zp-2 antibody
    • ZP2 antibody
    • ZP2_HUMAN antibody
    • ZPA antibody
    see all

Anti-ZP2 antibody images

  • Anti-ZP2 antibody (ab118073) at 5 µg/ml + Recombinant fragment of ZP2 with Proprietary tag at 0.2 µg

    Predicted band size : 82 kDa

References for Anti-ZP2 antibody (ab118073)

ab118073 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"