The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
FunctionThe mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Sequence similaritiesBelongs to the ZP domain family. ZPA subfamily. Contains 1 ZP domain.
Developmental stageExpressed during the 2-week growth phase of oogenesis, prior to ovulation.
DomainThe ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
Post-translational modificationsProteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. O-glycosylated; contains sulfate-substituted glycans.
Cellular localizationSecreted > extracellular space > extracellular matrix and Cell membrane.