Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab70384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
  • Tissue specificity
    Oocytes.
  • Sequence similarities
    Belongs to the ZP domain family. ZPC subfamily.
    Contains 1 ZP domain.
  • Domain
    The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • Post-translational
    modifications
    Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
    N-glycosylated.
    O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HUMZP3 antibody
    • Processed zona pellucida sperm-binding protein 3 antibody
    • Sperm receptor antibody
    • zona pellucida glycoprotein 3 (sperm receptor) antibody
    • Zona pellucida glycoprotein 3 antibody
    • zona pellucida glycoprotein 3A (sperm receptor) antibody
    • Zona pellucida glycoprotein ZP3 antibody
    • Zona pellucida protein C antibody
    • Zona pellucida sperm binding protein 3 precursor antibody
    • Zp-3 antibody
    • ZP3 372 antibody
    • ZP3 424 antibody
    • ZP3 474 antibody
    • ZP3 antibody
    • ZP3_HUMAN antibody
    • ZP3A antibody
    • ZP3A/ZP3B antibody
    • ZP3B antibody
    • ZPC antibody
    see all

Images

  • Anti-ZP3 antibody (ab70384) at 1 µg/ml + JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 17 kDa (possible cleavage fragment).

References

ab70384 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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