Anti-ZPBP1 antibody (ab97691)
Key features and details
- Rabbit polyclonal to ZPBP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZPBP1 antibody -
Description
Rabbit polyclonal to ZPBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 29-285 of Human ZPBP1 (NP_008940).
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Positive control
- WB: 293T, A431, H1299 or HeLaS3 cell lysate; IHC-P: SCC25 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100. |
Target
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Relevance
ZPBP1 is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida -
Cellular localization
Secreted -
Database links
- Entrez Gene: 654401 Cow
- Entrez Gene: 11055 Human
- Entrez Gene: 397194 Pig
- Omim: 608498 Human
- SwissProt: Q9BS86 Human
- SwissProt: Q29108 Pig
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Alternative names
- 4930486K01Rik antibody
- FLJ9322 antibody
- Iam38 antibody
see all
Images
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All lanes : Anti-ZPBP1 antibody (ab97691) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
12% SDS-PAGE. -
ab97691 at 1/100 dilution staining ZPBP1 in paraffin-embedded SCC25 xenograft tissue by immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97691 has been referenced in 2 publications.
- Minucci S & Venditti M New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality. Int J Mol Sci 23:N/A (2022). PubMed: 35563519
- Liu X et al. iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure. Clin Proteomics 15:27 (2018). PubMed: 30166971