Overview

  • Product name
  • Description
    Rabbit polyclonal to ZRANB1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 672-701 of Human ZRANB1

  • Positive control
    • K562 cell lysate. Mouse brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab103417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 81 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the peptidase C64 family.
    Contains 1 OTU domain.
    Contains 3 RanBP2-type zinc fingers.
  • Domain
    The OTU domain mediates the deubiquitinating activity.
    The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp762P2216 antibody
    • hTrabid antibody
    • Trabid antibody
    • TRAF-binding protein domain antibody
    • Ubiquitin thioesterase ZRANB1 antibody
    • Zinc finger Ran-binding domain-containing protein 1 antibody
    • ZRAN1_HUMAN antibody
    • ZRANB1 antibody
    see all

Images

  • Anti-ZRANB1 antibody (ab103417) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 81 kDa
  • ab103417, at 1/50 dilution, staining ZRANB1 by immunohistochemistry in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Anti-ZRANB1 antibody (ab103417) at 1/100 dilution + Mouse cerebellum tissue lysate at 35 µg

    Predicted band size : 81 kDa

References

ab103417 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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