1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionPositive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C64 family. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.
DomainThe OTU domain mediates the deubiquitinating activity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.