The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 81 kDa.
Use at an assay dependent dilution.
FunctionPositive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C64 family. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.
DomainThe OTU domain mediates the deubiquitinating activity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.