Anti-ZSCAN4 antibody (ab106646)

Overview

  • Product name
  • Description
    Rabbit polyclonal to ZSCAN4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid peptide from near the carboxy terminus of Human ZSCAN4 (NP_689890).

  • Positive control
    • Mouse lung tissue lysate; Rat lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab106646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.

Target

  • Function
    Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.
  • Sequence similarities
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • Cellular localization
    Nucleus. Chromosome > telomere.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35105 antibody
    • MGC126787 antibody
    • MGC126789 antibody
    • Zinc finger and SCAN domain containing 4 antibody
    • Zinc finger and SCAN domain containing protein 4 antibody
    • Zinc finger and SCAN domain-containing protein 4 antibody
    • Zinc finger protein 494 antibody
    • ZNF 494 antibody
    • ZNF494 antibody
    • ZSCA4_HUMAN antibody
    • ZSCAN4 antibody
    see all

Images

  • All lanes : Anti-ZSCAN4 antibody (ab106646) at 1 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 49 kDa

  • Immunohistochemistry analysis of ZSCAN4 expression in fromalin-fixed, paraffin-embedded rat lung tissue with ab106646 at 5 µg/ml.
  • Immunofluorescence of ZSCAN4 in Rat Lung cells using ab106646 at 20 ug/ml.

References

ab106646 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (SHEF3 hESC)
Loading amount
20 µg
Specification
SHEF3 hESC
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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